Transfection Reagents
Lonza Walkersville Cell Line Nucleofector Kit V
The Cell Line Nucleofector Kit V is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b System. This kit has been determined to be optimal for transfecting following cell types:32D3T3-L1pre-adA20A375A7r5AGSARPE-19B16-F10BA/F3 (DSMZ)C2C12C6Capan-1CHO [suspension]CHO-S [suspension] (Life Technologies)COS-1HaCaTHCT116HEK293HepG2HL-60HUV-EC-CJurkatK-562 (DSMZ)MCF7MDA-MB-231MDA-MB-468NB-4 (DSMZ)NCTC clone 929Neuro-2a [N2a]NG108-15P19PC-12PC-3RajiRamosRAW 264.7RBL-1S49Saos-2Schneider‘s Drosophila Line 2SH-SY5YSK-N-SHSK-OV-3SW480T/C-28 a2T/GHA-VSMCT-47DTHP-1U-2 OSVeroBenefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells Low-throughput transfection in single cuvette format
Lonza Walkersville Cell Line Optimization 4D-Nucleofector X Kit
The Cell Line Optimization 4D-Nucleofector X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X Unit.Benefits:Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experimentOptimal Nucleofection conditions determined in the 16-well Nucleocuvette Strips are transferable to the 100 μL single Nucleocuvette in the 4D-Nucleofector X UnitConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X UnitContent:0.675 mL Nucleofector Solution SE0.675 mL Nucleofector Solution SF0.675 mL Nucleofector Solution SG3 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0) 4 x 20 µL 16-well Nucleocuvette Strips
LGC GENOMICS LLC (Lucigen) TransforMax EC100D pir-116 Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Andwin Scientific FectoPRO DNA Transfection Kit
The FectoPRO Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount.
Lonza Walkersville Human T Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human T Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL), 25 Single use pipettes
BIOLINK LABORATORIES LLC FECTOPRO TRANSFEC REAGENT 10ML
+10mL FectoPRO boosterReagent:FectroPRO®Molecule Delivered:DNAApplications:BioproductionCell Types:Suspension CHO, HEK-293, and derivativesNumber of Transfections:1mL of FectoPRO® is sufficient to transfect 800mL to more than 1L of cell cultureStorage:5°C ± 3°C, stable for 1 to 2 years when stored appropriatelyProvided with:FectoPRO® Booster
Mayflower Bioscience LentiBOOST Research Grade Lentivirus Transduction Enhancer Solution, 200 Transductions
LentiBOOST is a chemical transduction enhancer simultaneously neutralizing electrostatic repulsion between cell membrane and virion and enhancing viral membrane fusion. In contrast to other chemical enhancers, LentiBOOST shows no toxic effects even in high concentrations. Therefore LentiBOOST is highly recommended for hard to transduce cells even in the presence of other transduction enhancers. LentiBOOST-R Research grade material is used for basic research only and is not transferable to the clinic. This is the latest technology which has been produced by Revvity and introduced by Mayflower Bioscience.